SRA

Clinical isolates of Photorhabdus asymbiotica have been recovered from patients in both the United States of America and Australia, and the full sequence of P. asymbiotica ATCC43949 from the USA has recently been reported. In contrast to other bacteria in the genus which only infect insects, P. asymbiotica strains are able to infect both insects and humans. Using a combination of Solexa (Illumina) and 454 Life Sciences (Roche) sequence data in different assembly pipelines, we report on a draft genome sequence of a strain of P. asymbiotica recovered from a patient from Kingscliff, Australia. The best assembly yielded an N50 scaffold size of 288,627 bp with >88.6% of the predicted genome covered by scaffolds over 100,000 bp. One of the central differences found between this Australian isolate and the USA isolate is the presence of an additional plasmid, pPAA2. This plasmid is similar to pCRY from Yersinia pestis, the causative agent of bubonic plague, and the presence of pPAA2 may account for the increased virulence of Australian isolates both against tissue culture cells and infected patients. The genome of the Kingscliff strain also contains several genomic differences from the USA isolate whose potential significance in virulence against both man and insects is discussed.